Description of Services
Design BAC modification strategy Once you tell us the changes you wish to make to your BAC, our skilled scientists will design a detailed strategy to achieve the modification. This can be as simple as telling us "I want to put a lacZ reporter after the promoter of this gene."
Leave BAC ordering to us Just give us the ID number of the BAC you want, and we can source it for you. The great majority of BACs are available from the BACPAC Resource Center at CHORI, but some BACs have to be obtained from other suppliers. This step is not needed if you send us your own BAC in E. coli.
|Design BAC modification strategy||1-4 days|
|Obtain BAC from vendor||1-2 weeks|
|One-step BAC modification (without removing drug-selection cassette)||2-4 weeks|
|Optional: Remove drug-selection cassette used in one-step BAC modification||1-2 weeks|
|Two-step BAC modification||4-8 weeks|
|Add drug-selection or visualization marker to BAC backbone**||1-2 weeks|
|Transfer region of BAC onto plasmid||2-4 weeks|
|Validation of modified BAC||2-3 days|
We guarantee successful BAC modification or we will fully refund your service fee – the best guarantee in the industry.
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We consider all reagents and animals resulting from our services to be the customers’ property. All information regarding our services, including customer identity and contact information, is kept confidential.