Viral Packaging
Virus Packaging
VectorBuilder, a sister company of Cyagen Biosciences, provides virus packaging services with rapid turnaround and unbeatable prices. We can also custom build viral vectors based on your research needs. Our viruses undergo strict quality control to ensure that they meet the purity, viability and titer requirements. Appropriate control viruses are also included in the final deliverables of custom virus packaging. Detailed description of our virus packaging services, including ordering information, is available on VectorBuilder's website, under Products & Services > Services > Virus Packaging.
Workflow of Virus Packaging Services
Types of Packaging Services Offered
Currently, VectorBuilder offers packaging services for the following virus types:
- Lentivirus (third generation)
- Adenovirus
- AAV (second generation), serotype 1, 2, 3, 4, 5, 6, 7, 8, 9, rh10
- MMLV
| Virus Type | Scale | Application | Titer & Volume | Turnaround |
Lentivirus |
Medium | Cell culture | >108 TU/ml, 1 ml | 7-14 days |
| Large | Cell culture | >109 TU/ml, 1 ml | 7-14 days | |
| Ultra-purified | In vivo | >109 TU/ml, 1 ml | 7-14 days | |
Adenovirus |
Medium | Cell culture | >1010 PFU/ml, 1 ml | 20-32 days |
| Large | Cell culture | >1011 PFU/ml, 1 ml | 20-32 days | |
| Ultra-purified | In vivo | >1012 VP/ml, 2 ml | 22-36 days | |
AAV |
Medium | Cell culture | >1011 GC/ml, 1 ml | 7-14 days |
| Large | Cell culture | >1012 GC/ml, 1 ml | 7-14 days | |
| Ultra-purified | In vivo | >1012 GC/ml, 1 ml | 9-16 days | |
MMLV |
Medium | Cell culture | >107 TU/ml, 1 ml | 7-14 days |
| Large | Cell culture | >108 TU/ml, 1 ml | 7-14 days | |
| Ultra-purified | In vivo | >108 TU/ml, 1 ml | 7-14 days |
Guarantee
For virus packaging services, our titer guarantee only applies to vectors for which the region being packaged into virus is below the recommended upper range (see table below). For sizes over this range, it may still be possible to package the vector into virus, but the titer may be reduced. Additionally, the guarantee does not apply to vectors which express genes that adversely affect the packaging process such as toxic genes.
| Virus Type | Upper Limit of Viral Genome | Upper Limit of User’s DNA Fragment |
| Lentivirus | 9.2 kb (from △5’ LTR to △U3/3’ LTR) | 6.4 kb |
| Adenovirus | 38.7 kb (from 5’ LTR to 3’ LTR) | 7.5 kb |
| AAV | 4.7 kb (from 5’ LTR to 3’ LTR) | 4.2 kb |
| MMLV retrovirus | 8 kb (from 5’ MoMuLV LTR to 3’ MoMuLV LTR) | 5.5 kb |