Viral Packaging

Virus Packaging

VectorBuilder, a sister company of Cyagen Biosciences, provides virus packaging services with rapid turnaround and unbeatable prices. We can also custom build viral vectors based on your research needs. Our viruses undergo strict quality control to ensure that they meet the purity, viability and titer requirements. Appropriate control viruses are also included in the final deliverables of custom virus packaging. Detailed description of our virus packaging services, including ordering information, is available on VectorBuilder's website, under Products & Services > Services > Virus Packaging.

Workflow of Virus Packaging Services

Types of Packaging Services Offered

Currently, VectorBuilder offers packaging services for the following virus types:

  • Lentivirus (third generation)
  • Adenovirus
  • AAV (second generation), serotype 1, 2, 3, 4, 5, 6, 7, 8, 9, rh10
  • MMLV
Virus Type Scale Application Titer & Volume Turnaround

Lentivirus

Medium Cell culture >108 TU/ml, 1 ml 7-14 days
Large Cell culture >109 TU/ml, 1 ml 7-14 days
Ultra-purified In vivo >109 TU/ml, 1 ml 7-14 days

Adenovirus

Medium Cell culture >1010 PFU/ml, 1 ml 20-32 days
Large Cell culture >1011 PFU/ml, 1 ml 20-32 days
Ultra-purified In vivo >1012 VP/ml, 2 ml 22-36 days

AAV

Medium Cell culture >1011 GC/ml, 1 ml 7-14 days
Large Cell culture >1012 GC/ml, 1 ml 7-14 days
Ultra-purified In vivo >1012 GC/ml, 1 ml 9-16 days

MMLV

Medium Cell culture >107 TU/ml, 1 ml 7-14 days
Large Cell culture >108 TU/ml, 1 ml 7-14 days
Ultra-purified In vivo >108 TU/ml, 1 ml 7-14 days

Guarantee

For virus packaging services, our titer guarantee only applies to vectors for which the region being packaged into virus is below the recommended upper range (see table below). For sizes over this range, it may still be possible to package the vector into virus, but the titer may be reduced. Additionally, the guarantee does not apply to vectors which express genes that adversely affect the packaging process such as toxic genes.

Virus Type Upper Limit of Viral Genome Upper Limit of User’s DNA Fragment
Lentivirus 9.2 kb (from △5’ LTR to △U3/3’ LTR) 6.4 kb
Adenovirus 38.7 kb (from 5’ LTR to 3’ LTR) 7.5 kb
AAV 4.7 kb (from 5’ LTR to 3’ LTR) 4.2 kb
MMLV retrovirus 8 kb (from 5’ MoMuLV LTR to 3’ MoMuLV LTR) 5.5 kb